Statistical evaluation of the tissue response to the root end filling materials indicated that no significant differences existed at the P < 0.05 level (Table 2) between the Diaket or MTA root-end filling materials in terms of the designated histological parameters (Table 1). In particular, the following were observed.
- No significant difference was found between MTA and Diaket for the presence of inflammation or abscess formation (Table 2).
- The greater amount of bone formation in the body of the wound was associated with the Diaket root-end filling cases. However, there was nostatistically significant difference between the materials (Table 2). The degree of new bone formation in the body of the excisional wounds was relatively complete with both materials (Figs 1and 2).
- New periodontal ligament formation (Fig. 2) was greater, adjacent to the Diaket root-end filling materials; however, there was no statistically significant difference (Table 2).
- MTA demonstrated more new bone formation adjacent to the root-end filling than did Diaket. Once again however, there was no statistically significant difference (Table 2).
- The presence of a complete cemental covering of the root apex and filling material was variable and unpredictable with both materials. Some excellent examples of new cementum-like formation over the root-end filling material were observed for both materials (Figs 2-5).
A wide range of responses was observed with some sections showing little inflammatory cell infiltrate, whilst others presented with a marked inflammatory cell presence.
Table 2. Results of statistical analysis.
Figure 1. A 60-day MTA section showing significant periodontal architecture regeneration in the surgical site adjacent to the root-end fill (REF), cementum-like material (arrows). Haematoxylin and eosin (x20).
Figure 2. A 60-day Diaket specimen showing extensive regeneration of all component of the periodontal architecture adjacent to the resected root end and root-end filling. Masson's Trichrome (x40). B, bone; PDL, periodontal ligament; REF, root-end filling; D, dentin.
Figure 3. A 60-dayMTA specimen showing extensive regeneration of all components of the periodontal architecture adjacent to the resected root end and root-end filling. Masson's Trichrome (x40). B, bone; PDL, periodontal ligament; REF, root-end filling; D, dentine. Cementum-like material (arrows) in intimate contact with the root-end filling material.
Figure 4. Masson's Trichrome specimen demonstrating cementum-like material (C) adjacent to the Diaket root-end filling and newly formed periodontal ligament (x100).
Figure 5. A x400 view of the cementum-like material interposed between the MTA root-end filling material and the newly formed periodontal ligament. Masson's Trichrome stain.