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Azerbaycan Saytlari

 »  Home  »  Endodontic Articles 11  »  Cytotoxicity of dentine-bonding agents on human pulp cells in vitro
Cytotoxicity of dentine-bonding agents on human pulp cells in vitro
Results - Discussion - References.

The results showed that dentine-bonding agents were cytotoxic to primary human pulp cells by MTT assay. The sensitivity of cytotoxicity to human pulp cells depended on the materials tested.
As shown in Fig. 1, eluates from five dentine-bonding agents were cytotoxic to primary human pulp cells (P < 0.05). SB was significantly more cytotoxic than the other dentine-bonding agents tested. Day 2 eluates from all materials tested significantly inhibited growth of cells (P < 0.05), but the leaching of toxic substances diminished at Day 5 (Fig.1). In addition, the Day 5 eluates of CB and HB on pulp cells were not significantly different from control values (P > 0.05).
In general, the rank orders with respect to cytotoxicity were found to be as follows: SB > PB > SC > HB > CB.

Figure 1. Effect of the eluates from five dentine-bonding agents on human pulp cells by MTT assay. Percentage of absorbance from each material, compared with that of control was calculated.

Effect of the eluates from five dentine-bonding agents on human pulp cells by MTT assay

In this study, cultured human pulp cells were used to evaluate the cytotoxicity of dentine-bonding agents. Primary pulp cells are more closely related to their original tissue and much easier to identify. In addition, pulp cells have a nearly unchanged metabolic state relative to their original tissue; the in vitro experiments thus approximate the in vivo situation. This is the reason why primary human pulp cells were chosen in this study.
Under normal conditions, few pulp cells proliferate in pulp tissue (Fitzgerald 1979). Pulp cells in the resting stage seem to reflect the in vivo condition more closely than do cells in the growing phase. Thus, in the present study, the cytotoxicity of dentine-bonding agents were examined on confluent cultures according to our recent studies (Chang & Chou 2001, Huang & Chang 2002b).
Several dentine-bonding agents have been evaluated for their cytotoxic effects (Hashieh et al. 1999, Schedle et al.1998, de SouzaCosta et al.1999, Kaga et al.2001, Szep et al. 2002, Huang & Chang 2002a). In accordance with this study, all dentine-bonding agents proved to be cytotoxic on different cell culture systems. Substances leached from dentine-bonding materials may be the reason why these materials exhibit cytotoxic effects. It has been demonstrated that unconverted monomers can be released from resin into an adjacent aqueous phase (Gerzina & Hume 1996) and can diffuse through dentine to the pulp space (Gerzina & Hume 1994). The toxic potential of components from dentine-bonding agents has been shown in vitro. Constituents of dentine- bonding agents, such as bisphenol-A-glycidyl methacrylate (Bis-GMA), triethyleneglycol dimethacrylate (TEGDMA) and urethane dimethacrylate (UDMA), were found to leach from dentine-bonding agents and may cause adverse effects (Geurtsen et al. 1998). It was found that hydrophilic monomers such as 2-hydroxyethyl methacrylate (HEMA) or TEGDMA were cytotoxic but to a lesser grade than the more hydrophobic monomers Bis-GMA or UDMA (Ratanasthien et al. 1995). In addition, HEMA or TEGDMA may also have an influence on the immune system (Rakich et al.1999).
In addition, dentine-bonding agents were found to release photoinitiator camphoroquinone (CQ) (Geursten et al. 1999). CQ is one of the photosensitizers, a widely used aliphatic type, which has been found attributed to free radicals including reactive oxygen generation (Atsumi et al. 1998). It also has been documented not only as acytotoxic agent (Atsumi et al.1998, Geursten et al. 1999) but also as a mutagen (Heil et al. 1996). Thus, CQ leached from dentine-bonding agents may partly explain why dentine-bonding agents are toxic agents.
From the results observed in the present study, it is concluded that dentine-bonding agents are cytotoxic to human pulp cells. However, the dentine might be important in protecting the dental pulp from the effects of substances leached from dentine-bonding agents. It is difficult to determine how much leach able substances actually act on pulp cells after diffusing through the dentine. Furthermore, the pulp circulation in vivo will wash out and reduce the concentrations of cytotoxic agents on pulp cells. Because dentine-bonding agents come into close and prolonged contact with vital dentine, comprehensive studies should be undertaken to clarify the substances leached from dentine-bonding agents effects pulp tissue both in vitro and in vivo.


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