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Azerbaycan Saytlari

 »  Home  »  Endodontic Articles 2  »  Cytotoxicity of resin-, zinc oxide–eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells
Cytotoxicity of resin-, zinc oxide–eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells
Results.



The results showed that resin-based, zinc oxide–eugenolbased, and calcium hydroxide-based root canal sealers were cytotoxic to primary human PDL cultures and V79 cells by MTT assay. The sensitivity of toxicity depended on the materials tested and the cell culture system used. Figures 1 and 2 show the cytotoxic effects of three different sealers on PDL cells and V79 cells, respectively.
The six sealers clearly induced cytotoxic effects. N2 was significantly more cytotoxic ( P < 0.05) than the other sealers in both cultures (Figs 1, 2). As shown in Figs 1 and 2, Sealapex initially resulted in a good biocompatibility to PDL cells or V79 cells compared with the control ( P > 0.05). Day 1 elutes of AH26 and AHPlus significantly inhibited growth of cells, but the leaching of toxic substances diminished from day 2 (Figs 1, 2). Extracts from day 1 through 3 of AH26 and AHPlus inhibited viability of PDL significantly more in comparison to V79 cells ( P < 0.05), whereas the day 7 elution of AH26 and AHPlus on PDL cells was not significantly different from control values (Fig. 2).

Effect of elutes of three types of root canal sealer on V79 cells by MTT assay. Percentage of absorbance at each elute compared with that of control was calculated
Figure 1. Effect of elutes of three types of root canal sealer on V79 cells by MTT assay. Percentage of absorbance at each elute compared with that of control was calculated. Each bar represents a mean SD. *, ** denote significant differences from control values with P <0.05 and P <0.001, respectively.

Effect of elutes of three types of root canal sealer on human PDL cells by MTT assay
Figure 2. Effect of elutes of three types of root canal sealer on human PDL cells by MTT assay. Percentage of absorbance at each elute compared with that of control was calculated. Each bar represents a mean SD. *, ** denote significant differences from control values with P <0.05 and P <0.001, respectively.

Canals and Endomethansone also demonstrated cytotoxic effects on both cultures (Figs 1, 2). However, human PDL cells were more sensitive to Canals than V79 cells ( P < 0.05). In contrast, Endomethansone significantly inhibited V79 cell viability compared to PDL cells ( P < 0.05).
As shown in Fig. 1, the reduction of cell viability from the final extracts of Sealapex was not significantly different from control values in both cultures. In addition, the final extracts of Canals, AHPlus, and AH26 did not exert cytotoxic effects on PDL cells (Fig. 2).
In general, the rank order for both cultures demonstrated the same pattern. The rank orders with respect to cytotoxicity were found to be as follows: N2 > Endomethansone > AH26 > AHPlus > Canals > Sealapex.