Article Options
Categories


Search


Advanced Search



This service is provided on D[e]nt Publishing standard Terms and Conditions. Please read our Privacy Policy. To enquire about a licence to reproduce material from endodonticsjournal.com and/or JofER, click here.
This website is published by D[e]nt Publishing Ltd, Phoenix AZ, US.
D[e]nt Publishing is part of the specialist publishing group Oral Science & Business Media Inc.

Creative Commons License


Recent Articles RSS:
Subscribe to recent articles RSS
or Subscribe to Email.

Blog RSS:
Subscribe to blog RSS
or Subscribe to Email.


Azerbaycan Saytlari

 »  Home  »  Endodontic Articles 2  »  Developmental changes and regional differences in histochemical localization of hyaluronan and versican in postnatal molar dental pulp
Developmental changes and regional differences in histochemical localization of hyaluronan and versican in postnatal molar dental pulp
Results.



At day 1, HA staining (Fig. 1a) and immunostaining for 12C5 (Fig. 1b) were found throughout in the interior parts of the mandibular first molar dental pulp.
At day 7, HA staining was found throughout the dental pulp, but a strong staining area appeared in the subodontoblastic layer (Fig. 2a), whilst immunostaining for 12C5 was still restricted to the interior dental pulp (Fig. 2b).
At day 14, the mandibular first molar had already finished its crown formation. HA staining (Fig. 3a) and immunostaining for 12C5 (Fig. 3b) were found throughout the dental pulp, but strong reactions for both molecules were seen in the subodontoblastic layer.
At day 21, the molar had erupted and its root formation had started. Figure 4a,c,e shows HA staining and Fig. 4b,d,f shows immunostaining for 12C5 cut longitudinally (Fig. 4a,b), and transversely through the coronal pulp (Fig. 4c,d) and the radicular pulp (Fig. 4e,f ). Although strong reactions for both HA and 12C5 were seen in the subodontoblastic layer of the coronal pulp (Fig. 4a–d), a strong reaction for HA and a weak reaction for 12C5 were seen in the subodontoblastic layer of the radicular pulp (Fig. 4a,b,e,f ). A strong reaction for 12C5 gradually shifted centripetally with increasing distance from the coronal pulp toward the root (see Fig. 4b). Immunostaining for CS-56 showed an identical staining pattern to 12C5 at this stage (Fig. 4g) and at all other ages examined (data not shown). These results confirmed versican localization in the corresponding area.

Serial sections showing HA staining (a) and immunostaining for 12C5 antibodies
Figures 1, 2, 3. Serial sections showing HA staining (a) and immunostaining for 12C5 antibodies (b). Bars = 100 m. At day 1 postnatal, uniform staining patterns for both molecules are seen in the interior parts of the dental pulp (arrow in 1a,b). At day 7, a strong reaction for HA is seen in the subodontoblastic layer (arrow in 2a), whereas the reactivity for versican is still restricted to the interior pulp (arrow in 2b). At day 14, the tooth crown formation has almost finished and strong reactions for both molecules are seen in the subodontoblastic layer (arrows in 3a,b).

At days 28 and 35, the staining pattern for both molecules was similar to that at day 21 (data not shown).
At day 42, although the subodontoblastic layer in the coronal pulp still showed strong reactions for both molecules (Fig. 5a–d), a 12C5-negative area first appeared in the central pulp region (Fig. 5a,c). In the corresponding area, HA staining was detectable but weak (Fig. 5b,d). Perfusion-fixation sections showed similar results (data not shown), indicating these findings were not fixation artifacts. Meanwhile, the staining pattern in the radicular pulp for either molecule did not change (Fig. 5a,b).
At day 49, the 12C5-negative, low-HA area in the central coronal pulp expanded (Fig. 6a,b).
No immunostaining was seen in the negative control section at day 21 (Fig. 7a). Similarly, positive reactions for HA completely disappeared after digestion with the Streptomyces hyalurolyticus hyaluronidase in the negative control section (Fig. 7b). Similarly, none of the negative control sections showed positive reactions for immunostaining or HA staining at any stage (data not shown).