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Azerbaycan Saytlari

 »  Home  »  Endodontic Articles 7  »  The effect of AH 26 and AH Plus on MCF-7 breast cancer cell proliferation in vitro
The effect of AH 26 and AH Plus on MCF-7 breast cancer cell proliferation in vitro
Results.



The addition of 10 pm estradiol-17b to CDHuS-supplemented medium increased the numbers of MCF-7 cells in culture 4.7 _0.2-fold over control (Table 1) (P < 0.01). In the absence of estradiol-17b (control) cells proliferated minimally.
The six samples of endodontic sealers prepared as described previously were assayed. Results are shown in Table 1. The three samples prepared with AH Plus
  1. paste A (epoxy resin and others);
  2. paste B (amine and others); and
  3. mixed AH Plus pastes A/B 1:1, did not induce MCF-7 cell proliferation (P > 0.05).
Cell toxicity was observed at 1/100 dilution of paste A - AH Plus, and at 1/1000 dilution of paste B-AH Plus and mixed AH Plus.
On the contrary, the sample prepared with powder of AH 26 inducedMCF-7 cell proliferation in a dose-dependent manner (Fig. 1). The cell yield obtained with AH 26-powder at1/100 dilution sample was 2.5-fold greater than in control cultures (P < 0.05).The sample prepared with mixed AH 26 paste/powder 1 :1 also induced MCF-7 cell proliferation (P < 0.05) (Fig. 2), but showed less potency than AH 26-powder alone. The cell yield obtained with AH 26 paste/powder at 1/100 dilution sample was 1.9-fold greater than in control cultures (P < 0.05). The paste of the endodontic sealer AH 26 alone did not stimulate MCF-7 cell proliferation (P > 0.05), and caused cell toxicity at 1/1000 dilution (Table 1).

Table 1. Cell proliferation of MCF-7 cells in the presence of different substances.

Cell proliferation of MCF-7 cells in the presence of different substances

Figure 1. Oestrogenic effect of AH 26-powder. Values significantly different from control, *P <0.05.

Oestrogenic effect of AH 26-powder. Values significantly different from control

Figure 2. Oestrogenic effect of AH 26-mixture. Values significantly different from control, *P <0.05.

MOestrogenic effect of AH 26-mixture. Values significantly different from control