The addition of 10 pm estradiol-17b to CDHuS-supplemented medium increased the numbers of MCF-7 cells in culture 4.7 _0.2-fold over control (Table 1) (P < 0.01). In the absence of estradiol-17b (control) cells proliferated minimally.
The six samples of endodontic sealers prepared as described previously were assayed. Results are shown in Table 1. The three samples prepared with AH Plus
- paste A (epoxy resin and others);
- paste B (amine and others); and
- mixed AH Plus pastes A/B 1:1, did not induce MCF-7 cell proliferation (P > 0.05).
Cell toxicity was observed at 1/100 dilution of paste A - AH Plus, and at 1/1000 dilution of paste B-AH Plus and mixed AH Plus.
On the contrary, the sample prepared with powder of AH 26 inducedMCF-7 cell proliferation in a dose-dependent manner (Fig. 1). The cell yield obtained with AH 26-powder at1/100 dilution sample was 2.5-fold greater than in control cultures (P < 0.05).The sample prepared with mixed AH 26 paste/powder 1 :1 also induced MCF-7 cell proliferation (P < 0.05) (Fig. 2), but showed less potency than AH 26-powder alone. The cell yield obtained with AH 26 paste/powder at 1/100 dilution sample was 1.9-fold greater than in control cultures (P < 0.05). The paste of the endodontic sealer AH 26 alone did not stimulate MCF-7 cell proliferation (P > 0.05), and caused cell toxicity at 1/1000 dilution (Table 1).
Table 1. Cell proliferation of MCF-7 cells in the presence of different substances.
Figure 1. Oestrogenic effect of AH 26-powder. Values significantly different from control, *P <0.05.
Figure 2. Oestrogenic effect of AH 26-mixture. Values significantly different from control, *P <0.05.