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Azerbaycan Saytlari

 »  Home  »  Endodontic Articles 8  »  Comparative in-vitro evaluation of three chelator pastes
Comparative in-vitro evaluation of three chelator pastes
Results.



Changes of microhardness Regardless of working time (2.5,5,10 min) no significant differences between the three chelator pastes were found. Mean loss of hardness was 4.4% for Calcinase- Slide after 2.5 min, 10.0% after 5 min and 21.2% after 10 min working time. The results for RC-Prep were 5.6% (2.5 min), 11.5% (5 min) and 18.6% (10 min) and for Glyde-File 5.5% (2.5 min), 11.1% (5 min) and 15.1% (10 min). For each of the pastes microhardness decreased significantly with increasing working time (Kruskal- Wallis test, P-values for all chelator pastes and working times <0.0013). Compared to the controls (mean loss of microhardness: 1%) the results for the chelating agents differed significantly regardless of working time (all P-values <0.0022) (Fig. 3).

Figure 3. Differences in microhardness before and after use of a chelating paste (in percentage of preoperative value). For each paste there was a significant decrease in microhardness with increasing working time and when compared to the respective controls.

Differences in microhardness before and after use of a chelating paste

Figure 4. Differences in weight of dentine slices before and after treatment with a chelator paste (in percentage of preoperative weight). For each of the pastes there were no significant differences in weight loss with regard to different working times of the pastes. After 6 and 9 min Calcinase resulted in a significant higher weight loss than RC-Prep, after 6 min Glyde-File removed significantly more dentine than RC-Prep.

Differences in weight of dentine slices before and after treatment with a chelator paste

Figure 5. Results for root-canal cleanliness after use of Calcinase-Slide. The best results were achieved in the coronal and middle parts of the root canal. For the coronal part the results were significantly better than for Glyde-File, RC-Prep and the control group, in the middle part significantly better than for the control group.

Results for root-canal cleanliness after use of Calcinase-Slide

Figure 6. Results for root-canal cleanliness after use of RC-Prep.

Results for root-canal cleanliness after use of RC-Prep

Figure 7. Results for root-canal cleanliness after use of Glyde-File.

Results for root-canal cleanliness after use of Glyde-File

Figure 8. Root-canal wall (middle third) after use of Calcinase-Slide (magnification1000x). Cleanliness was rated score I.

oot-canal wall (middle third) after use of Calcinase-Slide

Figure 9. Root-canal wall (middle third) after use of RC-Prep (magnification 1000x). Cleanliness was rated score I.

Root-canal wall middle third after use of RC-Prep

Figure 10. Root-canal wall (middle third) after use of Glyde-File (magnification1000x). Cleanliness was rated score I.

Cleanliness was rated score

Loss of weight.
The results for the loss of weight after different working times of the chelator pastes are summarized in Fig. 4. The highest amount of weight loss was observed for an application time of 3 min for Calcinase- Slide (0.3%) and RC-Prep (0.3%), for Glyde-File an application time of 6 min resulted in the greatest weight loss (0.25%).
For all three chelating agents the differences between the three working times were not statistically significant (Kruskal-Wallis test, Calcinase-Slide P ј 0.89, Glyde-File P ј 0.29, RC-Prep P ј 0.08).
After 3 min no significant differences were found between the groups, after 6- and 9-min specimens treated with Calcinase-Slide showed a significantly higher weight loss than specimens treated with RC-Prep (Wilcoxon-Mann-Whitney test, P ј 0.0089 and P ј 0.0271), after 6 min Glyde-File removed significantly more substance than RC-Prep (P ј 0.0108). The differences between all experimental groups and the control group were statistically significant for all working times.

Canal cleanliness.
The results of the SEM evaluation of root-canal cleanliness are presented in Figs 5-7. For all groups root-canal cleanliness decreased from the coronal to the apical third. Whereas in the coronal part a high number of scores I and II could be found (Figs 8-10), scores III and IV dominated in the apical third. The Kruskal-Wallis test showed significant differences for the coronal and middle parts of the root canals (P ј 0.0007 and P ј 0.0289, respectively). For these two sections, pair wise comparisons between the groups were performed using the Wilcoxon-Mann-Whitney test with P < 0.05. In the coronal third of the root canals the results for Calcinase- Slide showed statistically significant differences when compared to the other chelator pastes (Glyde-File: P ј 0.0024, RC-Prep: P ј 0.0002) and the control group (P ј 0.0051). In the middle part of the root canals, the results for Calcinase-Slide were significantly better than for the control group (P ј 0.0310) and for Glyde-File (P ј 0.0269). In the apical part no significant differences between the four groups existed.